Development of a dinoflagellate-oriented PCR primer set leads to detection of picoplanktonic dinoflagellates from Long Island Sound.
نویسندگان
چکیده
We developed dinoflagellate-specific 18S rRNA gene primers. PCR amplification using these oligonucleotides for a picoplanktonic DNA sample from Long Island Sound yielded 24 clones, and all but one of these clones were dinoflagellates primarily belonging to undescribed and Amoebophrya-like lineages. These results highlight the need for a systematic investigation of picodinoflagellate diversity in both coastal and oceanic ecosystems.
منابع مشابه
Specific growth rate and mitotic index in dinoflagellate Symbiodinium sp. isolateed from sea anemone Stichodactyla haddoni
The cultivation techniques of dinoflagellates is often problematic due to their sensitivity to hydrodynamic (shear) stress. For this study, sea anemone was collected from the east coast of Hormuz Island. First, we extracted symbiotic dinoflagellate, Symbiodinium sp. from Stichodactylla haddoni using manually homogenization. After transferring to the laboratory, samples were cultured in differen...
متن کاملHigh-level diversity of dinoflagellates in the natural environment, revealed by assessment of mitochondrial cox1 and cob genes for dinoflagellate DNA barcoding.
DNA barcoding is a diagnostic technique for species identification using a short, standardized DNA. An effective DNA barcoding marker would be very helpful for unraveling the poorly understood species diversity of dinoflagellates in the natural environment. In this study, the potential utility for DNA barcoding of mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob) was assessed. ...
متن کاملOligonucleotide Primers for the Detection of Bioluminescent Dinoflagellates Reveal Novel Luciferase Sequences and Information on the Molecular Evolution of This Gene(1).
Bioluminescence is reported in members of 18 dinoflagellate genera. Species of dinoflagellates are known to have different bioluminescent signatures, making it difficult to assess the presence of particular species in the water column using optical tools, particularly when bioluminescent populations are in nonbloom conditions. A "universal" oligonucleotide primer set, along with species and gen...
متن کاملDevelopment of a Multiplex Polymerase Chain Reaction for Differential Diagnosis of Canary Pox Virus
Background and Aims: A multiplex transcription-polymerase chain reaction (m-PCR) was developed for direct detection and discrimination between canarypox virus (CPV) and other avian poxvirus (APV). Materials and Methods: Three compatible primer sets were designed for m-PCR amplification of different loci fpv126, fpv140, and fpv167 located at highly conserved APV genes. Results: Results showed th...
متن کاملA Simple Genome Walking Strategy to Isolate Unknown Genomic Regions Using Long Primer and RAPD Primer
Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 72 8 شماره
صفحات -
تاریخ انتشار 2006